Kupanga njira yosalunjika ya ELISA yodziwira matenda a porcine pachimake kutsekula m'mimba coronavirus IgG antibody yotengera mapuloteni a spike

Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) ndi porcine enteric pathogenic coronavirus yomwe yangopezeka kumene yomwe imatha kuyambitsa matenda otsekula m'mimba mwa ana a nkhumba ongobadwa kumene ndikuwonongeka kwakukulu kwachuma kumakampani a nkhumba.Pakalipano, palibe njira yoyenera ya serological yowunikira mphamvu ya matenda a SADS-CoV ndi katemera, kotero pakufunika mwachangu kugwiritsa ntchito ma enzyme-linked immunosorbent assay (ELISA) kuti athetse vutoli.Apa, plasmid yophatikizanso yowonetsa puloteni ya SADS-CoV spike (S) yosakanikirana ndi domeni yaumunthu ya IgG Fc idapangidwa kuti ipange recombinant baculovirus ndikuwonetseredwa m'maselo a HEK 293F.Puloteni ya S-Fc imayeretsedwa ndi mapuloteni a G ndipo imasungabe mphamvu ndi anti-anthu Fc ndi anti-SADS-CoV ma antibodies.Kenako mapuloteni a S-Fc adagwiritsidwa ntchito popanga ELISA (S-iELISA) ndikusintha momwe S-iELISA imayendera.Zotsatira zake, popenda mtengo wa OD450nm wa 40 SADS-CoV sera yolakwika yotsimikiziridwa ndi immunofluorescence assay (IFA) ndi Western blotting, mtengo wodulidwa unatsimikiziridwa kukhala 0.3711.Coefficient of variation (CV) ya sera yabwino ya 6 SADS-CoV mkati ndi pakati pa S-iELISA zonse zinali zosakwana 10%.Mayeso a cross-reactivity adawonetsa kuti S-iELISA alibe cross-reactivity ndi sera ina ya porcine virus.Kuphatikiza apo, potengera kuzindikira kwa zitsanzo za seramu zachipatala za 111, kuchuluka kwangozi kwa IFA ndi S-iELISA kunali 97.3%.Mayeso a virus neutralization okhala ndi 7 osiyanasiyana OD450nm ma seramu adawonetsa kuti mtengo wa OD450nm wodziwika ndi S-iELISA udalumikizidwa bwino ndi mayeso a virus neutralization.Potsirizira pake, S-iELISA inachitidwa pa zitsanzo za seramu za nkhumba za 300.Zida zamalonda za ma enterovirus ena a nkhumba zidawonetsa kuti IgG zabwino za SADS-CoV, TGEV, PDCoV ndi PEDV zinali 81.7%, 54%, ndi 65.3%, motsatana., 6%, motero.Zotsatira zikuwonetsa kuti S-iELISA ndi yeniyeni, yokhudzidwa, komanso yobereka, ndipo ingagwiritsidwe ntchito pozindikira matenda a SADS-CoV mumsika wa nkhumba.Nkhaniyi ndi yotetezedwa ndi kukopera.maumwini onse ndi otetezedwa.