Vanessa Chen

Jiangsu Konsung Bio-Medical Science and Technology Co., Ltd. Nanjing 210012, Jiangsu, China

Chidule: Anemia, matenda ofala kwambiri padziko lonse lapansi, amakhudza kwambiri thanzi ndi moyo wa anthu mamiliyoni mazanamazana. Kuyang'ana koyambirira ndikofunikira kwambiri pakuwongolera matenda a odwala komanso kuchepetsa kuchuluka kwa matenda. Popeza kuti chithandizo chamankhwala chikutsika kwambiri, ndikofunikira kupanga zida zowunikira zolondola komanso zosavuta. Nkhaniyi ikutenga Konsung Hemoglobin Analyzer (mndandanda wa H7) ngati chinthu chofufuzira ndikuwunika momwe amagwiritsira ntchito mfundo yake yodziwikiratu pakuwunika magazi. Kupyolera mu kusanthula mwadongosolo kagwiritsidwe ntchito ka zida, deta yotsimikizira zachipatala zambiri, ndi kuyerekeza ndi njira zoyesera zama labotale, zidapezeka kuti chipangizochi chikuwonetsa kulondola kwambiri (± 2% zolakwika zosiyanasiyana) komanso kuchita bwino kwambiri (3 masekondi/zitsanzo) pakuzindikira kuchuluka kwa hemoglobin. Mapangidwe ake osunthika komanso njira yosavuta yopangira opaleshoni idatsitsira kwambiri ogwira ntchito zachipatala kuti azigwiritsa ntchito. Ndizoyenera makamaka kuwunika kwakukulu m'mabungwe oyambira azachipatala. Kafukufukuyu adawonetsanso kuti chipangizochi chili ndi chiwerengero choyambirira chowunika cholondola cha 92.3% cha kuchepa kwa magazi m'thupi, chikhoza kuzindikira bwino magulu omwe ali pachiopsezo chachikulu, kupereka maziko odalirika a matenda ndi chithandizo chamankhwala, ndipo ali ndi zofunikira zachipatala ndi chikhalidwe cha anthu pakulimbikitsa kusintha kwa patsogolo kwa kupewa kuchepa kwa magazi m'thupi ndi chithandizo ndi kupeza matenda oyambirira ndi chithandizo.

Mawu osakira: Hemoglobin analyzer; Kuyeza magazi m'thupi; Spectrophotometry; Konsung H7; Kulondola kwa matenda

Mawu Oyamba

Kuperewera kwa magazi m'thupi ndi matenda a hemoglobin (HB) otsika kwambiri, omwe amapezeka padziko lonse lapansi pafupifupi 24.8%, omwe kusowa kwachitsulo kumayambitsa 50%. Kuperewera kwa magazi m'thupi kwa nthawi yayitali sikumangopangitsa kuti chitetezo cha mthupi chifooke komanso kusokonezeka kwa chidziwitso, komanso kungayambitse matenda amtima ndi cerebrovascular, makamaka kuyambitsa

chiopsezo chachikulu kwa magulu apadera monga amayi apakati ndi ana. Komabe, kuwunika kwachikhalidwe, komwe kumadalira kuyezetsa magazi nthawi zonse ndipo kumafuna zida zazikulu zodzipangira okha, kumakhala ndi zovuta monga kukwera mtengo kwa zida, ntchito yovuta, komanso kuyezetsa kwanthawi yayitali, zomwe zimapangitsa kuti zikhale zovuta kukwaniritsa kufunikira kozindikira mwachangu kuchipatala choyambirira [1]. Konsung hemoglobin analyzer (H7 series) yayamba kutchuka pang'onopang'ono pakuwunika magazi m'mabungwe oyambira azachipatala chifukwa cha kusuntha kwake komanso mawonekedwe ake ozindikira mwachangu (nthawi yozindikira ≤ masekondi 3). Chipangizochi chimagwiritsa ntchito ma spectrophotometry apamwamba kuti adutse luso lazodziwika bwino. Ikhoza kukwaniritsa kuzindikirika kolondola ndi magazi ochepa omwe amasonkhanitsidwa kuchokera ku chala, kuwongolera kwambiri kuyang'anira bwino komanso kutsata kwa odwala. Nkhaniyi imayang'ana mwadongosolo momwe chipangizochi chimagwirira ntchito komanso kufunika kwake kwachipatala, ndicholinga chofuna kupereka maziko asayansi kuti athe kuwongolera mapulogalamu owunika magazi m'thupi komanso kulimbikitsa chithandizo chamankhwala chokhazikika, ndikuthandizira kuthana ndi vuto la kuchepa kwa chithandizo chamankhwala pamlingo woyamba.

Makhalidwe aukadaulo a Konsung hemoglobin analyzer

Kuzindikira mfundo ndi magawo apakati

Chipangizochi, chotengera mfundo ya spectrophotometer, chimayesa kuchuluka kwa hemoglobin m'magazi athunthu pogwiritsa ntchito njira ya SLS-Hb, yokhala ndi 0-250g/L ndi 1g/L [2]. Zigawo zake zazikuluzikulu zimaphatikizapo chipangizo cha microfluidic, gwero la kuwala kwa 532nm wavelength ndi ma photoelectric sensors, omwe amawerengera zomwe zili mu HB poyesa kuyamwa kwa kuwala kwa yankho pambuyo pa hemolysis. Kutsimikizika kwachipatala kunawonetsa kulumikizana kwa 0.98 (P

Ubwino wogwirira ntchito ndi zolephera

Ubwino wake

1. Kufulumira komanso kosavuta: Palibe chifukwa chopangira mankhwala ovuta, 7μL yokha ya magazi a chala ndiyofunikira kuti mutsirize kuyesa, kupulumutsa 90% ya nthawi yotolera zitsanzo poyerekeza ndi zoyezetsa mwazi zachizolowezi. Chidacho chili ndi mawonekedwe osavuta komanso owoneka bwino, ndipo pambuyo pa maphunziro okhazikika, ogwira ntchito zachipatala omwe si akatswiri amatha kumaliza mayesowo mumphindi imodzi, ndikuwongolera bwino pakuwunika [3]. Mwachitsanzo, pakuwunika thupi la okalamba m’deralo, kuchuluka kwa mayeso atsiku ndi tsiku kumatha kuwirikiza katatu kuposa zida za makolo.
2. Kutsika mtengo: Mtengo wa zinthu zomwe zimagwiritsidwa ntchito poyesa kamodzi ndi 30% zotsika kusiyana ndi zoyezetsa magazi nthawi zonse, ndipo zipangizozi ndizosavuta kuzisamalira ndipo sizifuna kuyesedwa kawirikawiri.
3. Kagwiritsidwe ntchito kachipangizo: Chipangizochi ndi chophatikizika komanso chosunthika, makamaka choyenera pazochitika zomwe zili ndi zinthu zochepa monga malo owerengera, malo osungira magazi, magalimoto operekera magazi, ndi zipatala zapagulu [4]. Pakufunsana kwaulere kwachipatala kumadera akutali kapena kuwunika zaumoyo kwa ogwira ntchito m'mabizinesi, mawonekedwe ake osafunikira magetsi akunja (okhala ndi batire yamkati yomwe imatha maola opitilira 8) imathetsa bwino vuto la zida zachikhalidwe zomwe zimadalira malo a labotale ndikulimbikitsa kufalikira kwa ntchito zowunika kuchepa kwa magazi m'magawo apansi ndi mafoni.

Kulumikizana kwa data

Yokhala ndi mphamvu yotumizira ma Bluetooth, imaphatikizana ndi nkhokwe zachipatala za m'madera kuti zigawane zenizeni zenizeni. Othandizira azaumoyo oyambira amatha kukweza mwachangu zotsatira kuzipatala zapamwamba kapena nsanja zaumoyo wa anthu, kuthandizira kupanga mbiri yaumoyo wa odwala komanso kuyang'anira mwamphamvu. Kusanthula kwakukulu kwa data kumathandiziranso maulamuliro azaumoyo kuti azindikire njira zochepetsera magazi m'dera kuti adziwitse njira zopewera kupewa [5].

Zolepheretsa

1. Zambiri za morphological zikusowa: Kulephera kupereka magawo ofunikira monga kuchuluka kwa maselo ofiira (MCV) ndi kufalikira kwa maselo ofiira (RDW), zomwe zimakhudza kuzindikira mitundu ya kuchepa kwa magazi;
2. Zosokoneza: Hyperbilirubinemia (> 20mg/dL) kapena zitsanzo za magazi a chylous zingayambitse zotsatira zokondera.

Kupambana kwatsopano pakuwunika kwa hemoglobin yonyamula

Ukadaulo waukadaulo wodziwikiratu

Hemoglobin ndi molekyu ya puloteni yomwe imapezeka m'maselo ofiira a magazi omwe amanyamula mpweya kuchokera m'mapapo kupita ku ziwalo zina za thupi. Ndilofunika pa moyo wa munthu. Kuchepa kwa hemoglobini kungasonyeze kuti munthu ali ndi matenda enaake, kuphatikizapo kuchepa kwa magazi m'thupi, khansa, matenda a impso, ndi cirrhosis, choncho kuyang'anira hemoglobini yeniyeni ndikofunikira. The Konsung H7 mndandanda kunyamula hemoglobin analyzer amagwiritsa microfluidic, spectrophotometry, ndi kumwaza chipukuta ukadaulo kuonetsetsa zachipatala molondola (CV ≤ 1.5%) [6-8]. 7μL yokha yamagazi a chala iyenera kusonkhanitsidwa, ndipo zotsatira zoyesa zidzawonetsedwa pazithunzi zamtundu wa TFT mkati mwa masekondi atatu. Kusungidwa kwa zotsatira za mayeso 2,000 kumapereka mwayi waukulu wowunika kuchuluka kwa hemoglobin m'moyo watsiku ndi tsiku.

Pogwiritsa ntchito njira zodalirika za hemoglobini ndi njira zowonjezera zogwiritsira ntchito zitsanzo, Hb ndi HCT zimatha kudziwika mofulumira komanso molondola kuti azindikire ndi kuyang'anira matenda monga kuchepa kwa magazi, polycythemia, sickle cell disease, etc. [9-11]. Amagwiritsidwa ntchito kwambiri m'malo opangira magazi ndipo atha kugwiritsidwanso ntchito kuchipatala, kuyezetsa pafupi ndi bedi, ma laboratories azachipatala, zipatala, ndi zina.

Kusanthula zochitika zachipatala

1. Kuwunika kwakukulu: Pakati pa mayeso a thupi a 5,000 omwe amachitidwa m'mabungwe oyambirira azachipatala, chipangizochi chinapeza chiwerengero chabwino cha 12.3% (616 milandu) cha kuchepa kwa magazi m'thupi, chomwe chinali chogwirizana ndi 91.8% ya zotsatira zowunikanso zotsatila zamagazi;
2. Kuwunika kwamphamvu: Kutsata kwa chithandizo cha iron supplementation kwa odwala omwe ali ndi vuto la kuchepa kwa magazi m'thupi kunawonetsa kuti kusintha kwa HB komwe kunapezeka kunali kogwirizana ndi kusintha kwazizindikiro zachipatala (r = 0.79, P
3. Kuyenerera kwa anthu ena: Kwa odwala ana (3-12 wazaka), palibe tsankho lazaka zomwe zidawonedwa pakulakwitsa koyesa (P = 0,45), koma chidwi chiyenera kuperekedwa ku chikoka cha mgwirizano wa ana pa nthawi yosonkhanitsa magazi.

Sensitivity ndi mwachindunji

Sankhani zitsanzo zamagazi a 800 (kuphatikiza zitsanzo za amuna 220, zitsanzo za akazi 300, ndi zitsanzo za ana 280) [12]. Mayeso akuchitika pano pa BC-5140 automatic cell analyzer yopangidwa ndi Mindray Bio-Medical Electronics Co., LTD. (pambuyo pake amatchedwa "BC-5140") ndiyeno pa Konsung H7-3 hemoglobin analyzer (yotchedwa "H7-3"), ndipo zotsatira za mayesero onsewa zinafufuzidwa mozama komanso kukhudzidwa ndi kutsimikizika kwa H7-3 hemoglobin analyzer [13]. Table 1 ikuwonetsa zotsatira za mayeso omwe amachitidwa pa zitsanzo za amuna. Pogwiritsa ntchito deta iyi, chidwi ndi tsatanetsatane zinawerengedwa, zomwe ndi 96.70% ndi 94.74%, motero.

Table 1. Mayeso a amuna

Kumverera = 176 / (176 + 6) × 100% = 96.70%

Kukhazikika = 36 / (36 + 2) × 100% = 94.74%

Table 2 amasonyeza zotsatira za mayeso ochitidwa pa zitsanzo za akazi. Pogwiritsa ntchito deta iyi, chidwi ndi tsatanetsatane zinawerengedwa, zomwe ndi 96.80% ndi 92.00%, motero.

Table 2. Deta ya mayeso achikazi

Kumverera = 242 / (242 + 8) × 100% = 96.80%

Kukhazikika = 46 / (46 + 4) × 100% = 92.00%

Table 3 amasonyeza zotsatira za mayeso ochitidwa pa zitsanzo zotengedwa kwa ana. Pogwiritsa ntchito deta iyi, chidziwitso ndi tsatanetsatane zinawerengedwa, zomwe ndi 98.33% ndi 85.00%, motero.

Table 3. Ana amayesa deta

Kumverera = 236 / (236+4) × 100% = 98.33%

Kukhazikika = 34 / (34 + 6) × 100% = 85.00%

Table 4 ikuwonetsa zotsatira za data yonse yoyeserera. The tilinazo ndi specifications Konsung H7-3 hemoglobin analyzer anali 97.32% , ndipo mwachindunji anali 90,63%.

Table 4. Zonse zoyesa mayeso

Sensitivity = 654 / (654+18) × 100% = 97.32%

Kukhazikika = 116 / (116+12) × 100% = 90.63%

Njira zothetsera khalidwe

Konsung imapereka pulogalamu yowongolera bwino yomwe idapangidwa kuti iwunikire ndikutsimikizira zotulukapo za mayeso opangidwa ndi H7 hemoglobin analyzer. Pulogalamuyi ili ndi:

1. Njira yothetsera khalidwe la hemoglobini: HBC-3A (Level L, M, H)
2. Chip chowongolera khalidwe la hemoglobini: HBQC-3C (Chip-high, Chip-katikati, Chip-low).

Kuyerekeza ndi Njira Zina Zodziwira

Njira ya Microscopic

Njira ya microscopic imalola kuzindikiritsa mawonekedwe a maselo ofiira a magazi, monga maselo omwe akulowera. Komabe, zimatenga nthawi, zomwe zimafuna kupitilira mphindi 15 pachitsanzo chilichonse, ndipo kulondola kwake kumadalira luso la wogwiritsa ntchitoyo komanso zomwe wakumana nazo.

Full automatic analyzer

Makina osanthula a hematology okhazikika amapereka kusanthula kwatsatanetsatane kwa maselo amwazi mwatsatanetsatane. Komabe, mtengo wawo wokwera, kuyambira nthawi 20 mpaka 50 kuposa zida za Konsung, zitha kukhala zolepheretsa kugwiritsidwa ntchito kwambiri.

Chida chonyamula cha photoelectric colorimetric

Zida zonyamulikazi zikuwonetsa magwiridwe antchito ofanana ndi osanthula a hemoglobin a Konsung H7. Komabe, pofika 2025, alibe ziphaso kuchokera ku National Medical Products Administration (NMPA), zomwe zitha kuletsa

kukhazikitsidwa kwachipatala ndi kuvomerezedwa ndi malamulo.

Malingaliro okhathamiritsa ndi ziyembekezo zamtsogolo

Kusintha kwaukadaulo

Integrated AI image recognition module for the semi-quantitative analysis of red blood cell morphology polumikizana ndi maikulosikopu akunja [14]. Pakadali pano, AI yawonetsa kuthekera kwakukulu pantchito yowunikira zithunzi zachipatala. Kuika m'maselo a hemoglobini kungathe kuzindikira kukula kwachilendo ndi mawonekedwe a maselo ofiira a magazi, zomwe zimathandiza madokotala kuzindikira mwamsanga zizindikiro za matenda monga kuchepa kwa iron kuperewera kwa magazi ndi megaloblastic anemia. Poyerekeza ndi kuwunika kwapang'onopang'ono kwachikale, AI imatha kuchepetsa zolakwika zongoganiza chabe, kukonza magwiridwe antchito komanso kulondola, ndipo ndiyoyenera makamaka pazochitika zomwe chithandizo choyambirira chachipatala sichikwanira.

Mayeso amitundu yambiri

Mayesero monga serum ferritin ndi transferrin saturation amapereka zidziwitso zofunikira pakusungira chitsulo ndi momwe amayendera, zomwe zimathandiza kusiyanitsa bwino pakati pa mitundu yosiyanasiyana ya kuchepa kwa magazi. Kudalira kokha kuyesa kwa hemoglobini nthawi zambiri sikukwanira, chifukwa sikusiyanitsa pakati pa zifukwa monga kusowa kwachitsulo, matenda aakulu, kapena matenda ena a hematologic [15]. Mwa kukulitsa zizindikiro zoyesa, chithandizo chamitundu yambiri chothandizira matenda a kuchepa kwa magazi m'thupi chimatha kupangidwa, kuthandiza madokotala kuzindikira chomwe chimayambitsa ndikukhazikitsa mapulani amunthu payekha. Izi sizingowonjezera kuchuluka kwa chithandizo choyambirira, komanso kuchepetsa kuchuluka kwa otumiza odwala ndikukwaniritsa kugawa kwamankhwala.

Mapeto

Konsung hemoglobin analyzer ikuwonetsa zabwino komanso zachuma pakuwunika koyambirira kwa kuchepa kwa magazi m'thupi ndipo ikhoza kukhala chida chokondedwa cha chisamaliro choyambirira. Pali kuchepa kwa zida zoyezera komanso akatswiri azachipatala m'mabungwe oyambira azachipatala. Zida zamakono zoyesera magazi m'thupi sizongovuta kuti zigwire ntchito, komanso zimakhala ndi zofunika kwambiri zachilengedwe ndi ogwira ntchito, zomwe zimapangitsa kuti zikhale zovuta kuchita ntchito yowunika mwamsanga. Konsung hemoglobin analyzer, komabe, imagwiritsa ntchito njira yabwino yosonkhanitsira magazi m'chala, sikutanthauza kusamalidwa bwino kwa zitsanzo, ndipo imatha kuyendetsedwa mwachangu ndi ogwira ntchito zachipatala pambuyo pa maphunziro osavuta, kufupikitsa njira yoyezetsa komanso kuwongolera bwino pakuwunika. Pazachuma, mtengo wa zida ndi zogwiritsira ntchito ndizotsika kwambiri kuposa zida zoyesera zachikhalidwe, zomwe zimatha kuchepetsa bwino ndalama zamabungwe azachipatala oyambira. Kuphatikiza apo, chipangizochi ndi chophatikizika komanso chosunthika, ndipo chimatha kusinthidwa kuti chizigwiritsidwa ntchito tsiku ndi tsiku, kuyang'anira mafoni kumadera akutali, ndikuyang'anira thanzi la anthu ammudzi, kupereka yankho lothandiza pakuwunika koyambirira kwa kuchepa kwa magazi m'munsi ndikuthandizira kukulitsa kuchuluka kwa kupewa kuchepa kwa magazi m'thupi komanso chithandizo chamankhwala pamlingo wapansi.

Chidziwitso chowululira

Wolembayo akunena kuti palibe kusagwirizana kwa chidwi.

Maumboni

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